DNA

Part:BBa_K2814022

Designed by: Saksham Sharma   Group: iGEM18_IIT_Delhi   (2018-10-10)


attB-BxbI (Reverse of BBa_K2814018 )

Contains attB site of Bxb1 integrase in the reverse orientation (Reverse of BBa_K2814018). Bxb1 integrase carries out site-specific recombination by catalyzing unidirectional recombination to produce AttL and AttR sites from AttP and AttB. This switching capacity allows them to be used in the design of logic gates.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 42
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 22


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Categories
Parameters
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